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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-85371 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GAD67 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (96%), Rat (96%), Dog (99%), Cat (99%), Pig (99%), Chicken (91%), Chimpanzee (100%), Bovine (98%). Positive Control: SK-N-SH, mouse brain, rat brain, human GAD1-transfected 293T. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.17 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GAD67 in various tissue extracts using GAD67 polyclonal antibody (Product # PA5-85371) using 50 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG and ECL secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GAD67 in A) 293T whole cell lysate, B) whole cell lysate using GAD67 polyclonal antibody (Product # PA5-85371) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GAD67 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a GAD67 Polyclonal Antibody (Product # PA5-85371) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GAD67 Polyclonal Antibody (Product # PA5-85371). Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 Polyclonal Antibody (Product # PA5-85371) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GAD67 Polyclonal Antibody (Product # PA5-85371). Mouse tissue extract (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 Polyclonal Antibody (Product # PA5-85371) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GAD67 Polyclonal Antibody detects GAD67 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD67 protein stained by GAD67 Polyclonal Antibody (Product # PA5-85371) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta-3 Tubulin Polyclonal Antibody [GT886]diluted at 1:500. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GAD67 Polyclonal Antibody detects GAD67 protein expression by immunohistochemical analysis. Sample: Paraffin-Embedded adult mouse retina. Green: GAD67 protein stained by GAD67 Polyclonal Antibody (Product # PA5-85371) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GAD67 Polyclonal Antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GAD67 stained by GAD67 Polyclonal Antibody (Product # PA5-85371) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GAD67 Polyclonal Antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GAD67 stained by GAD67 Polyclonal Antibody (Product # PA5-85371) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
