Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM20216PU-N - Provider product page
- Provider
- OriGene
- Product name
- GSK3 alpha (GSK3A) pTyr278/215 (incl. pos. control) mouse monoclonal antibody, clone 6D3, Purified
- Antibody type
- Monoclonal
- Description
- GSK3 alpha (GSK3A) pTyr278/215 (incl. pos. control) mouse monoclonal antibody, clone 6D3, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GSK3A
- Isotype
- IgG
- Antibody clone number
- 6D3
- Vial size
- 100 µg
- Concentration
- NULL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Detection of endogenous GSK3b pTyr215, GSK3a pTyr278: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20216PU-N GSK3alpha/beta antibody (Clone 6D3) at 0.5 ug/ ml for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12; Lane 7: CMT 93 Lane 8: Neuro 2A Lane 9: 3T3
- Validation comment
- WB