AM20216PU-N

antibody from OriGene

Targeting: GSK3A

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AM20216PU-N

Provider

OriGene

Product name

GSK3 alpha (GSK3A) pTyr278/215 (incl. pos. control) mouse monoclonal antibody, clone 6D3, Purified

Antibody type

Monoclonal

Description

GSK3 alpha (GSK3A) pTyr278/215 (incl. pos. control) mouse monoclonal antibody, clone 6D3, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

GSK3A

Isotype

IgG

Antibody clone number

6D3

Vial size

100 µg

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Detection of endogenous GSK3b pTyr215, GSK3a pTyr278: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20216PU-N GSK3alpha/beta antibody (Clone 6D3) at 0.5 ug/ ml for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12; Lane 7: CMT 93 Lane 8: Neuro 2A Lane 9: 3T3

Validation comment

WB