Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB12623 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12623, RRID:AB_10549930
- Product name
- GSK3A (phospho S21) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of GSK3A.
- Antigen sequence
RTSSFA- Storage
- Store at 4°C. For long term storage store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Submitted references GSK3alpha exhibits beta-catenin and tau directed kinase activities that are modulated by Wnt.
Protein kinase C betaII regulates Akt phosphorylation on Ser-473 in a cell type- and stimulus-specific fashion.
Asuni AA, Hooper C, Reynolds CH, Lovestone S, Anderton BH, Killick R
The European journal of neuroscience 2006 Dec;24(12):3387-92
The European journal of neuroscience 2006 Dec;24(12):3387-92
Protein kinase C betaII regulates Akt phosphorylation on Ser-473 in a cell type- and stimulus-specific fashion.
Kawakami Y, Nishimoto H, Kitaura J, Maeda-Yamamoto M, Kato RM, Littman DR, Leitges M, Rawlings DJ, Kawakami T
The Journal of biological chemistry 2004 Nov 12;279(46):47720-5
The Journal of biological chemistry 2004 Nov 12;279(46):47720-5
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The NIH/3T3 cell lysate derived from conditioned culture medium was resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with GSK3A (phospho S21) polyclonal antibody (Cat # PAB12623) at 1 : 500 (Lane 1). Lane 2 was pre-incubated with immunizing peptide.
