MA5-15301

antibody from Invitrogen Antibodies

Targeting: GSK3A

ELISA

Antibody data

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Validation data

Product number: MA5-15301 - Provider product page
Provider: Invitrogen Antibodies
Product name: GSK3 alpha Monoclonal Antibody (6G2F1, 6G2F2)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15301 targets GSK3 alpha in indirect ELISA, IHC and WB applications and shows reactivity with Human samples. The MA5-15301 immunogen is purified recombinant fragment of GSK3 alpha expressed in E. Coli.
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: 6G2F1, 6G2F2
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

GSK3A protein structure - MA5-15301 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GSK3 alpha using a GSK3 alpha monoclonal antibody (Product # MA5-15301) against a truncated GSK3 alpha recombinant protein (1) and A549 cell lysate (2).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of GSK3A was achieved by transfecting GSK3A with GSK3A specific siRNAs (Silencer® select Product # s6236,). Western blot analysis (Fig. a) was performed using whole cell extracts from the GSK3A knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with GSK3 alpha Monoclonal Antibody (6G2F1, 6G2F2) (Product # MA5-15301, 1:1000 dilution) and Goat Anti-Mouse IgG Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to GSK3A.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-GSK3 alpha Monoclonal Antibody (6G2F1, 6G2F2) (Product # MA5-15301) and a 51 kDa band corresponding to GSK3A, along with an uncharacterized band (*) at ~45kDa was observed across the cell lines tested. Whole cell extracts (40 µg lysate) of A549 (Lane 1), HeLa (Lane 2), MDA-MB-231 (Lane 3), COS-7 (Lane 4), tissue extracts of Mouse brain (Lane 5) and Rat testis (Lane 6)were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).