Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- R1471P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1471P, RRID:AB_1001933
- Product name
- anti GSK3 alpha pSer21
- Antibody type
- Polyclonal
- Antigen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 16-25 of human GSK3 alpha.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Western blot using Affinity Purifiedanti-GSK3A antibody shows detection of a 52 kDa bandcorresponding to human GSK3A present in ~ 35 µg ofHEK293 whole cell lysate (lane 1). A 4-20% TrisGlycine gel was used for separation followed byblocking with 5% BLOTTO in PBS. Primary antibodywas used at a 1:1,000 dilution in blocking buffer andreacted overnight at 4°C. The membrane was washedand reacted with a 1:10,000 dilution of IRDye 800TMconjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel,green). Molecular weight estimation was made bycomparison to prestained MW markers in lane M (700nm channel, red). IRDye TM800 fluorescence image wascaptured using the Odyssey® Infrared Imaging Systemdeveloped by LI-COR. IRDye is a trademark of LI-COR,Inc. Other detection systems will yield similar results.