AM20216PU-N

antibody from Acris Antibodies GmbH

Targeting: GSK3A

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AM20216PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM20216PU-N, RRID:AB_10759017

Product name

anti GSK3 alpha/beta pTyr278/215 (incl. pos. control)

Antibody type

Monoclonal

Antigen

Phosphopeptide conjugated to hemocyaninEpitope: Phosphotyrosine 215, Phosphotyrosine 278

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

6D3

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Detection of endogenous GSK3b pTyr215, GSK3a pTyr278: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20216PU-N GSK3alpha/beta antibody (Clone 6D3) at 0.5 µg/ ml for 1h at RT and developed by ECL (exp. time: 30 sec).Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5YLane 5: MDCK Lane 6: PC12; Lane 7: CMT 93 Lane 8: Neuro 2ALane 9: 3T3