Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AM20216PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM20216PU-N, RRID:AB_10759017
- Product name
- anti GSK3 alpha/beta pTyr278/215 (incl. pos. control)
- Antibody type
- Monoclonal
- Antigen
- Phosphopeptide conjugated to hemocyaninEpitope: Phosphotyrosine 215, Phosphotyrosine 278
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6D3
- Vial size
- 0.1 mg
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Detection of endogenous GSK3b pTyr215, GSK3a pTyr278: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20216PU-N GSK3alpha/beta antibody (Clone 6D3) at 0.5 µg/ ml for 1h at RT and developed by ECL (exp. time: 30 sec).Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5YLane 5: MDCK Lane 6: PC12; Lane 7: CMT 93 Lane 8: Neuro 2ALane 9: 3T3