A00971-1
antibody from Boster Biological Technology
Targeting: HDAC4
BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA, KIAA0288
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- A00971-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-HDAC4 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for Histone Deacetylase 4/HDAC4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, ICC/IF, FCM. Reactive species: Human;Mouse;Rat. Histone Deacetylase 4/HDAC4 information: Molecular Weight: 119040 MW; Subcellular Localization: Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4 and SIK1. The nuclear localization probably depends on sumoylation; Tissue Specificity: Ubiquitous.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of THP-1 cells using anti-HDAC4 antibody (A00971-1). Overlay histogram showing THP-1 cells stained with A00971-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- HDAC4 Antibody (A00971-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
- Additional image