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LearnA00971-1
antibody from Boster Biological Technology
Targeting: HDAC4
BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA, KIAA0288
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- A00971-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-HDAC4 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for Histone Deacetylase 4/HDAC4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, ICC/IF, FCM. Reactive species: Human;Mouse;Rat. Histone Deacetylase 4/HDAC4 information: Molecular Weight: 119040 MW; Subcellular Localization: Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4 and SIK1. The nuclear localization probably depends on sumoylation; Tissue Specificity: Ubiquitous.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Positive programming of the GC-IGF1 axis mediates adult osteoporosis susceptibility in male offspring rats induced by prenatal dexamethasone exposure.
Wilson Disease: Intersecting DNA Methylation and Histone Acetylation Regulation of Gene Expression in a Mouse Model of Hepatic Copper Accumulation.
Overexpression of miR-140 Inhibits Proliferation of Osteosarcoma Cells via Suppression of Histone Deacetylase 4.
Shangguan Y, Li X, Qin J, Wen Y, Wang H, Chen L
Biochemical pharmacology 2022 Dec;206:115264
Biochemical pharmacology 2022 Dec;206:115264
Wilson Disease: Intersecting DNA Methylation and Histone Acetylation Regulation of Gene Expression in a Mouse Model of Hepatic Copper Accumulation.
Sarode GV, Neier K, Shibata NM, Shen Y, Goncharov DA, Goncharova EA, Mazi TA, Joshi N, Settles ML, LaSalle JM, Medici V
Cellular and molecular gastroenterology and hepatology 2021;12(4):1457-1477
Cellular and molecular gastroenterology and hepatology 2021;12(4):1457-1477
Overexpression of miR-140 Inhibits Proliferation of Osteosarcoma Cells via Suppression of Histone Deacetylase 4.
Xiao Q, Huang L, Zhang Z, Chen X, Luo J, Zhang Z, Chen S, Shu Y, Han Z, Cao K
Oncology research 2017 Jan 26;25(2):267-275
Oncology research 2017 Jan 26;25(2):267-275
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of THP-1 cells using anti-HDAC4 antibody (A00971-1). Overlay histogram showing THP-1 cells stained with A00971-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- HDAC4 Antibody (A00971-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
- Additional image
