Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
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Validation data
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- Product number
- MA5-27522 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HOMER1 Monoclonal Antibody (OTI7G10)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI7G10
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HOMER1 in HepG2, HeLa, mouse brain, rat brain cells using 35 µg per lane. Samples were probed with HOMER1 (Product # MA5-27522) monoclonal antibody at a dilution of 1:500.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of HOMER1 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with HOMER1 (Product # MA5-27522) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of HOMER1 was achieved by transfecting IMR-32 with HOMER1 specific siRNAs (Silencer® select Product # S18117, S18118). Western blot analysis (Fig. a) was performed using whole cell lysate from the HOMER1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with HOMER1 Monoclonal Antibody (OTI7G10) (Product # MA5-27522, 1:500 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to HOMER1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-HOMER1 Monoclonal Antibody (OTI7G10) (Product # MA5-27522) and a 40kDa band corresponding to HOMER1 was observed across all cell lines and tissues tested except Mouse Kidney and Rat Kidney which are reported to be negative. Whole Cell Extract-WCL (30 µg lysate) of IMR-32 (Lane 1), SK-N-AS (Lane 2), Mouse Brain (Lane 3), Mouse Skeletal Muscle (Lane 4), Mouse Kidney (Lane 5), Rat Brain (Lane 6), Rat Skeletal Muscle (Lane 7) and Rat Kidney (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human embryonic cerebellum tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a HOMER1 monoclonal antibody (Product # MA5-27522) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human spleen tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a HOMER1 monoclonal antibody (Product # MA5-27522) at a dilution of 1:500.