PA5-21487

antibody from Invitrogen Antibodies

Targeting: HOMER1 HOMER-1B, SYN47, Ves-1

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-21487 - Provider product page
Provider: Invitrogen Antibodies
Product name: HOMER1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, mouse brain, rat brain. Predicted reactivity: Mouse (97%), Rat (91%), Xenopus laevis (85%), Pig (98%), Rhesus Monkey (99%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.11 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

HOMER1 protein structure - PA5-21487 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Homer-1b, c using 30 µg IMR32 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Homer-1b, c polyclonal antibody (Product # PA5-21487) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Homer-1b, c using 20 µg of mouse brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Homer-1b, c polyclonal antibody (Product # PA5-21487) at a dilution of 1:20,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Homer-1b, c using 50 µg rat brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Homer-1b, c polyclonal antibody (Product # PA5-21487) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Homer-1b, c using Various whole cell extracts (30 µg). Samples were loaded onto a 10% SDS-PAGE gel and probed with a Homer-1b, c polyclonal antibody (Product # PA5-21487) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of HOMER1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a HOMER1 Polyclonal Antibody (Product # PA5-21487) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using HOMER1 Polyclonal Antibody (Product # PA5-21487). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HOMER1 Polyclonal Antibody (Product # PA5-21487) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of HOMER1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a HOMER1 Polyclonal Antibody (Product # PA5-21487) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HOMER1 was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a HOMER1 Polyclonal Antibody (Product # PA5-21487) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Skeletal Muscle (Lane 3), Mouse Kidney (Lane 4), Mouse Lung (Lane 5), Mouse Spleen (Lane 6) and Rat Spleen (Lane 7). The blot was probed with Anti-HOMER1 Polyclonal Antibody (Product # PA5-21487, 1:3000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 43 kDa band corresponding to HOMER1 was observed in Mouse Brain, Rat Brain and Mouse Skeletal Muscle compared to other tissue extracts which are reported to be negative for HOMER1 expression. An additional band at 60 KDa was observed in Mouse Skeletal Muscle.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Homer-1b, c in methanol-fixed HeLa cells using a Homer-1b, c polyclonal antibody (Product # PA5-21487) at a 1:500 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of HOMER1 was performed in DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: HOMER1 Polyclonal Antibody (Product # PA5-21487) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of methanol-fixed HeLa, using Homer antibody (Product # PA5-21487) at 1:500 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of HOMER1 was performed using 90% confluent log phase PC-12 cells differentiated to Neuronal cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HOMER1 Rabbit Polyclonal Antibody (Product # PA5-21487) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytoplasmic localization. Panel e shows undifferentiated cells with reduced signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.