PA5-23023

antibody from Invitrogen Antibodies

Targeting: HOMER1 HOMER-1B, SYN47, Ves-1

Antibody data

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Validation data

Product number: PA5-23023 - Provider product page
Provider: Invitrogen Antibodies
Product name: HOMER1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: The target sequence has 99% sequence homology with rat, 98% to bovine, 96% to xenopus and 74% to zebrafish.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

HOMER1 protein structure - PA5-23023 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HOMER1 using a polyclonal antibody (Product # PA5-23023).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HOMER1 using a polyclonal antibody (Product # PA5-23023).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HOMER1 in mouse brain lysate. Sample was incubated in HOMER1 polyclonal antibody (Product # PA5-23023).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HOMER1 in human brain lysate. Sample was incubated in HOMER1 polyclonal antibody (Product # PA5-23023).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-HOMER1 Polyclonal Antibody (Product # PA5-23023) and a 40kDa band corresponding to HOMER1 was observed across in Mouse Brain, Mouse Skeletal Muscle, Rat Brain, Rat Skeletal Muscle but was not observed in Mouse Kidney, Mouse Spleen, Rat Kidney and Rat Spleen which are reported to be negative. Whole Cell Extract-WCL (30 µg lysate) of Mouse Brain (Lane 1), Mouse Skeletal Muscle (Lane 2), Mouse Kidney (Lane 3), Mouse Spleen (Lane 4), Rat Brain (Lane 5), Rat Skeletal Muscle (Lane 6), Rat Kidney (Lane 7), Rat Spleen (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).