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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- PA5-78327 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HOXB13 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: PC3 WCE, NE
- Concentration
- 1 mg/mL
Submitted references Long Noncoding RNA HOXA11-AS and Transcription Factor HOXB13 Modulate the Expression of Bone Metastasis-Related Genes in Prostate Cancer.
Misawa A, Kondo Y, Takei H, Takizawa T
Genes 2021 Jan 27;12(2)
Genes 2021 Jan 27;12(2)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HOXB13 in A) PC-3 whole cell extract, B) PC-3 nuclear extract using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with HOXB13 polyclonal antibody (Product # PA5-78327) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HOXB13 Polyclonal Antibody detects HOXB13 protein by western blot analysis. A. 30 µg PC-3 whole cell extract. B. 30 µg PC-3 nuclear extract.12 % SDS-PAGE. HOXB13 Polyclonal Antibody (Product # PA5-78327) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 IBSP promoter is directly regulated by HOXB13 in combination with HOXA11-AS . ( A ) IBSP expression analysis by qPCR in PC3 cells transfected for 48 h with siHOXB13 or siHOXA11-AS. ( B ) IBSP exons, promoter region with HOXB13 binding site, and luciferase vector insert are shown. ( C ) Luciferase assay of PC3 cells co-transfected with siHOXB13 or siHOXA11-AS and luciferase vector. Vectors with negative control siRNA (siNC) were used as controls. Values were normalized to Renilla luciferase activity. Data represent mean +- standard deviation. ( D ) Expression analysis of HOXA11-AS and IBSP by qPCR in PC3 cells transfected for 48 h with or without HOXA11-AS expression vector. ( E ) ChIP analysis of HOXB13 in IBSP promoter region in PC3 cells transfected for 24 h with siHOXA11-AS. IBSP promoter enrichment relative to input was measured by qPCR. qPCR analyses were performed in triplicate ( n = 3). Expression levels are presented relative to values of GAPDH , used as a reference gene. Values represent mean +- standard deviation. Statistical significance was determined by unpaired t -tests. *, p < 0.05. **, p < 0.01.
