Explore
Validate
LearnNBP2-22400
antibody from Novus Biologicals
Targeting: SERPINH1
CBP1, CBP2, colligen, HSP47, SERPINH2
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-22400 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Hsp47 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects 47kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.2 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Hsp47 Antibody (1C4-1A6) [NBP2-22400] - analysis of Human Epithelial cell (A431) lysates showing detection of 47 kDa Hsp47 protein using Mouse Anti-Hsp47 Monoclonal Antibody, Clone 1C4-1A6 . Lane 1: MW ladder. Lane 2: Anti-Hsp47 (1:250). Lane 3: Anti-Hsp47 (1:500). Lane 4: Anti-Hsp47 (1:1000). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody at 1:250 - 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: 47 kDa.
