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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX62628 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX62628, RRID:AB_10622430
- Product name
- HSP47 antibody [EPR4217]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Store at -20 ¢XC. Stable for 12 months from date of receipt.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis on (A) HeLa (B) A431 and (C) NIH/3T3 cell lysates using anti-HSP47 RabMAb (cat. #GTX62628).
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of (1) A431 and (2) NIH/3T3 lysates (20 £gg per lane) using HSP47 antibody [EPR4217] at a dilution of 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of human colon tissue using HSP47 antibody [EPR4217] at a dilution of 1:300. Antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Tissue counterstained with Hematoxylin.
