PA5-83072
antibody from Invitrogen Antibodies
Targeting: SERPINH1
CBP1, CBP2, colligen, HSP47, SERPINH2
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-83072 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SERPINH1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LSAFCLLEAA LAAEVKKPAA AAAPGTAEKL SPKAATLAER SAGLAFSLYQ AMAKDQAVEN ILVSPVVVAS SLGLVSLGGK ATTASQAKAV LSAEQLRDEE VHAGLGELLR SLSNSTARNV TWKL
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SERPINH1 by a SERPINH1 polyclonal antibody (Product # PA5-83072). Analysis in human cell line CACO-2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SERPINH1 by a SERPINH1 polyclonal antibody (Product # PA5-83072). Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Serpin H1 was achieved by transfecting Caco-2 with Serpin H1 specific siRNAs (Silencer® select Product # s2485, s2486). Western Blot analysis (Fig. a) was performed using Whole cell extracts from the Serpin H1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SERPINH1 Polyclonal Antibody (Product # PA5-83072, 0.1 µg/mL ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20000 dilution). Densitometric analysis of this Western Blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Serpin H1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-SERPINH1 Polyclonal Antibody (Product # PA5-83072) and a 47 kDa band corresponding to Serpin H1 was observed across tested cell lines. Whole cell extracts (40 µg lysate) of Caco-2 (Lane 1), NIH:OVCAR-3 (Lane 2), U-937 (Lane 3), Daudi (Lane 4), NIH/3T3 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SERPINH1 in U-2 OS cells using a SERPINH1 polyclonal antibody (Product # PA5-83072). The analysis shows positivity in endoplasmic reticulum.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Serpin H1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for overnight at room temperature. The cells were labeled with SERPINH1 Polyclonal Antibody (Product # PA5-83072) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing endoplasmic reticulum and cytoplasm localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SERPINH1 in human vulva/anal skin using a SERPINH1 polyclonal antibody (Product # PA5-83072). The analysis shows moderate cytoplasmic positivity in epidermal cells.