- PA5-27832 - Invitrogen Antibodies SERPINH1 antibody

Submitted references Small molecule inhibitor of HSP47 prevents pro-fibrotic mechanisms of fibroblasts in vitro.

Miyamura T, Sakamoto N, Kakugawa T, Taniguchi H, Akiyama Y, Okuno D, Moriyama S, Hara A, Kido T, Ishimoto H, Yamaguchi H, Miyazaki T, Obase Y, Ishimatsu Y, Tanaka Y, Mukae H
Biochemical and biophysical research communications 2020 Sep 24;530(3):561-565

Conditional deletion of Nedd4-2 in lung epithelial cells causes progressive pulmonary fibrosis in adult mice.

Duerr J, Leitz DHW, Szczygiel M, Dvornikov D, Fraumann SG, Kreutz C, Zadora PK, Seyhan Agircan A, Konietzke P, Engelmann TA, Hegermann J, Mulugeta S, Kawabe H, Knudsen L, Ochs M, Rotin D, Muley T, Kreuter M, Herth FJF, Wielpütz MO, Beers MF, Klingmüller U, Mall MA
Nature communications 2020 Apr 24;11(1):2012

Detection of RAGE expression and its application to diabetic wound age estimation.

Ji XY, Chen Y, Ye GH, Dong MW, Lin KZ, Han JG, Feng XP, Li XB, Yu LS, Fan YY
International journal of legal medicine 2017 May;131(3):691-698

Activation of α7nAChR Promotes Diabetic Wound Healing by Suppressing AGE-Induced TNF-α Production.

Dong MW, Li M, Chen J, Fu TT, Lin KZ, Ye GH, Han JG, Feng XP, Li XB, Yu LS, Fan YY
Inflammation 2016 Apr;39(2):687-99

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using SERPINH1 Polyclonal Antibody (Product # PA5-27832). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 10% SDS PAGE. SERPINH1 Polyclonal Antibody (Product # PA5-27832) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SERPINH1 Polyclonal Antibody detects HSP47 protein by western blot analysis. A. 30 µg A431 whole cell lysate/extract. B. 30 µg HeLa whole cell lysate/extract. C. 30 µg HepG2 whole cell lysate/extract.10% SDS-PAGE. SERPINH1 Polyclonal Antibody (Product # PA5-27832) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SERPINH1 Polyclonal Antibody detects HSP47 protein by western blot analysis. A. 30 µg U87-MG whole cell lysate/extract. B. 30 µg SK-N-SH whole cell lysate/extract. C. 30 µg IMR32 whole cell lysate/extract. D. 30 µg SK-N-AS whole cell lysate/extract.10% SDS-PAGE. SERPINH1 Polyclonal Antibody (Product # PA5-27832) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Serpin H1 was achieved by transfecting Caco-2 with Serpin H1 specific siRNAs (Silencer® select Product # s2485, s2486). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Serpin H1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SERPINH1 Polyclonal Antibody (Product # PA5-27832, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Serpin H1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-SERPINH1 Polyclonal Antibody (Product # PA5-27832) and a 47 kDa band corresponding to Serpin H1 was observed across tested cell lines with an uncharacterized band (*) at ~30 kDa. Whole cell extracts (40 µg lysate) of NIH:OVCAR-3 (Lane 1), Caco-2 (Lane 2), NIH/3T3 (Lane 3), U-937 (Lane 4), Daudi (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Serpin H1 was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SERPINH1 Polyclonal Antibody (Product # PA5-27832) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic (endoplasmic reticulum) localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SERPINH1 Polyclonal Antibody detects HSP47 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SERPINH1 Polyclonal Antibody (Product # PA5-27832) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SERPINH1 Polyclonal Antibody detects HSP47 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. SERPINH1 Polyclonal Antibody (Product # PA5-27832) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 7 Comparison of proteomic signatures of pulmonary fibrosis in conditional Nedd4-2 -/- mice and patients with IPF. a , b Heatmap of differentially regulated matrisome annotated proteins in lungs from conditional Nedd4-2 -/- mice ( n = 11) and control mice ( n = 13) induced with doxycycline for 3 months ( a ), and in lung tissues from IPF patients and controls ( n = 11/group) ( b ). c Venn-diagram showing proportion of unique and common differentially regulated proteins in lungs of conditional Nedd4-2 - /- mice and IPF patients. d Summary of differentially regulated matrisome proteins found in conditional Nedd4-2 - /- mice and human IPF samples. e Micrographs of representative lung sections from conditional Nedd4-2 - /- mice and littermate controls stained with anti-Tnc, anti-Col14a1, and anti-Serpinh1 antibodies ( n = 6/group). Scale bars, 100 um.

PA5-27832

Antibody data