Explore
Validate
Learn
Western blot
ELISA
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [2]
- Immunohistochemistry [3]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-118001 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IFNGR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IFNGR1 Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate, Lane B: 0.5 mg 293T Whole Cell Lysate, Lane C: 0.5 mg HepG2 Whole Cell Lysate 4 µL with IFNGR1 Polyclonal Antibody (Product # PA5-118001) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: IFNGR1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 100 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of IFNGR1 in Lane A: 0.5 mg HeLa Whole Cell Lysate, Lane B: 0.5 mg 293T Whole Cell Lysate, Lane C: 0.5 mg HepG2 Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-118001) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 54 kDa , Observed band size : 100 kDa .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) of IFNGR1 in human placenta. Samples were incubated with polyclonal antibody (Product # PA5-118001) with a dilution of 1:1,000 .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) of IFNGR1 in human kidney. Samples were incubated with polyclonal antibody (Product # PA5-118001) with a dilution of 1:1,000 .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human IFNGR1 in human placenta with IFNGR1 Polyclonal Antibody (Product # PA5-118001, 1:1,000 dilution, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of IFNGR1 in Lane A: 0.5 mg HeLa Whole Cell Lysate, Lane B: 0.5 mg 293T Whole Cell Lysate, Lane C: 0.5 mg HepG2 Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-118001) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 54 kDa , Observed band size : 100 kDa .
