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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB675-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IGFBP-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IGFBP-1 in ELISAs and Western blots. In Western blots, detection of recombinant mouse IGFBP-1 is observed but no cross-reactivity with recombinant human (rh) IGFBP-2, rhIGFBP-3, rhIGFBP-4 or rhIGFBP-5 is observered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Recombinant Human and Mouse IGFBP-1 by Western Blot. Western blot shows 25 ng of Recombinant Human IGFBP-1 (Catalog # 871-B1), Recombinant Mouse IGFBP-1 (Catalog # 1588-B1) and Recombinant Human IGFBP-5 (Catalog # 875-B5). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGFBP-1 Monoclonal Antibody (Catalog # MAB675) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGFBP-1 at approximately 26 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IGFBP-1 Inhibition of IGF-I-dependent Cell Proliferation and Neutralization by Human IGFBP-1 Antibody. Recombinant Human IGFBP-1 (Catalog # 871-B1) inhibits Recombinant Human IGF-I (Catalog # 291-G1) induced proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF-I (6 ng/mL) activity elicited by Recombinant Human IGFBP-1 (5 µg/mL) is neutralized (green line) by increasing concentrations of Human IGFBP-1 Monoclonal Antibody (Catalog # MAB675). The ND50 is typically 10-40 µg/mL.
