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- Antibody Data
- Antigen structure
- References [17]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB230-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-4R alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-4 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-9 R, recombinant mouse IL-4 R, rhIL-5 R alpha , rhIL-5 R beta , rhIL-13 R alpha 1, or rhIL-13 R alpha 2 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P24394- Isotype
- IgG
- Antibody clone number
- 25463
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Allergen-specific IgG antibody signaling through FcγRIIb promotes food tolerance.
IL4 Primes the Dynamics of Breast Cancer Progression via DUSP4 Inhibition.
IL-4 and IL-13 differentially regulate TLR-induced eosinophil-basophil differentiation of cord blood CD34+ progenitor cells.
Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1β-stimulated production of chemokines and matrix-degrading enzymes in vitro.
Cancer-initiating cells from colorectal cancer patients escape from T cell-mediated immunosurveillance in vitro through membrane-bound IL-4.
Absence of a role for interleukin-13 in inflammatory bowel disease.
Construction and application of elastin like polypeptide containing IL-4 receptor targeting peptide.
Platelet ITAM signaling is critical for vascular integrity in inflammation.
Hrs recognizes a hydrophobic amino acid cluster in cytokine receptors during ubiquitin-independent endosomal sorting.
Fatty acid amide hydrolase in prostate cancer: association with disease severity and outcome, CB1 receptor expression and regulation by IL-4.
Th2 cytokine-induced upregulation of 11beta-hydroxysteroid dehydrogenase-1 facilitates glucocorticoid suppression of proasthmatic airway smooth muscle function.
IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
Interleukin-4 induces the activation and collagen production of cultured human intrahepatic fibroblasts via the STAT-6 pathway.
Interleukin-4 induces human hepatocyte apoptosis through a Fas-independent pathway.
Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain.
A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
Burton OT, Tamayo JM, Stranks AJ, Koleoglou KJ, Oettgen HC
The Journal of allergy and clinical immunology 2018 Jan;141(1):189-201.e3
The Journal of allergy and clinical immunology 2018 Jan;141(1):189-201.e3
IL4 Primes the Dynamics of Breast Cancer Progression via DUSP4 Inhibition.
Gaggianesi M, Turdo A, Chinnici A, Lipari E, Apuzzo T, Benfante A, Sperduti I, Di Franco S, Meraviglia S, Lo Presti E, Dieli F, Caputo V, Militello G, Vieni S, Stassi G, Todaro M
Cancer research 2017 Jun 15;77(12):3268-3279
Cancer research 2017 Jun 15;77(12):3268-3279
IL-4 and IL-13 differentially regulate TLR-induced eosinophil-basophil differentiation of cord blood CD34+ progenitor cells.
Reece P, Gauvreau GM, Sehmi R, Denburg JA
PloS one 2014;9(6):e100734
PloS one 2014;9(6):e100734
Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1β-stimulated production of chemokines and matrix-degrading enzymes in vitro.
Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzì RM, Meliconi R
PloS one 2014;9(5):e96925
PloS one 2014;9(5):e96925
Cancer-initiating cells from colorectal cancer patients escape from T cell-mediated immunosurveillance in vitro through membrane-bound IL-4.
Volonté A, Di Tomaso T, Spinelli M, Todaro M, Sanvito F, Albarello L, Bissolati M, Ghirardelli L, Orsenigo E, Ferrone S, Doglioni C, Stassi G, Dellabona P, Staudacher C, Parmiani G, Maccalli C
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 1;192(1):523-32
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 1;192(1):523-32
Absence of a role for interleukin-13 in inflammatory bowel disease.
Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque SS, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza GR, MacDonald TT
European journal of immunology 2014 Feb;44(2):370-85
European journal of immunology 2014 Feb;44(2):370-85
Construction and application of elastin like polypeptide containing IL-4 receptor targeting peptide.
Sarangthem V, Cho EA, Bae SM, Singh TD, Kim SJ, Kim S, Jeon WB, Lee BH, Park RW
PloS one 2013;8(12):e81891
PloS one 2013;8(12):e81891
Platelet ITAM signaling is critical for vascular integrity in inflammation.
Boulaftali Y, Hess PR, Getz TM, Cholka A, Stolla M, Mackman N, Owens AP 3rd, Ware J, Kahn ML, Bergmeier W
The Journal of clinical investigation 2013 Feb;123(2):908-16
The Journal of clinical investigation 2013 Feb;123(2):908-16
Hrs recognizes a hydrophobic amino acid cluster in cytokine receptors during ubiquitin-independent endosomal sorting.
Amano Y, Yamashita Y, Kojima K, Yoshino K, Tanaka N, Sugamura K, Takeshita T
The Journal of biological chemistry 2011 Apr 29;286(17):15458-72
The Journal of biological chemistry 2011 Apr 29;286(17):15458-72
Fatty acid amide hydrolase in prostate cancer: association with disease severity and outcome, CB1 receptor expression and regulation by IL-4.
Thors L, Bergh A, Persson E, Hammarsten P, Stattin P, Egevad L, Granfors T, Fowler CJ
PloS one 2010 Aug 19;5(8):e12275
PloS one 2010 Aug 19;5(8):e12275
Th2 cytokine-induced upregulation of 11beta-hydroxysteroid dehydrogenase-1 facilitates glucocorticoid suppression of proasthmatic airway smooth muscle function.
Hu A, Fatma S, Cao J, Grunstein JS, Nino G, Grumbach Y, Grunstein MM
American journal of physiology. Lung cellular and molecular physiology 2009 May;296(5):L790-803
American journal of physiology. Lung cellular and molecular physiology 2009 May;296(5):L790-803
IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
The Journal of allergy and clinical immunology 2009 Jan;123(1):59-66.e4
The Journal of allergy and clinical immunology 2009 Jan;123(1):59-66.e4
Interleukin-4 induces the activation and collagen production of cultured human intrahepatic fibroblasts via the STAT-6 pathway.
Aoudjehane L, Pissaia A Jr, Scatton O, Podevin P, Massault PP, Chouzenoux S, Soubrane O, Calmus Y, Conti F
Laboratory investigation; a journal of technical methods and pathology 2008 Sep;88(9):973-85
Laboratory investigation; a journal of technical methods and pathology 2008 Sep;88(9):973-85
Interleukin-4 induces human hepatocyte apoptosis through a Fas-independent pathway.
Aoudjehane L, Podevin P, Scatton O, Jaffray P, Dusanter-Fourt I, Feldmann G, Massault PP, Grira L, Bringuier A, Dousset B, Chouzenoux S, Soubrane O, Calmus Y, Conti F
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 May;21(7):1433-44
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 May;21(7):1433-44
Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 1;169(9):5244-51
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 1;169(9):5244-51
Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain.
Hirst SJ, Hallsworth MP, Peng Q, Lee TH
American journal of respiratory and critical care medicine 2002 Apr 15;165(8):1161-71
American journal of respiratory and critical care medicine 2002 Apr 15;165(8):1161-71
A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
Journal of leukocyte biology 2001 Oct;70(4):617-23
Journal of leukocyte biology 2001 Oct;70(4):617-23
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-4 R alpha in HDLM-2 Human Cell Line. IL-4 R alpha was detected in immersion fixed HDLM-2 human Hodgkin's lymphoma cell line using Mouse Anti-Human IL-4 R alpha Monoclonal Antibody (Catalog # MAB230) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-4 R alpha in Human Lymph Node. IL-4 R alpha was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human IL-4 R alpha Monoclonal Antibody (Catalog # MAB230) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-4 R alpha in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL-4 R alpha Monoclonal Antibody (Catalog # MAB230, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-4 and Neutralization by Human IL-4 R alpha Antibody. Recombinant Human IL-4 (Catalog # 204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-4 R alpha Monoclonal Antibody (Catalog # MAB230). The ND50 is typically 3-6 ng/mL.
