14-1249-82

antibody from Invitrogen Antibodies
Targeting: IL4R CD124
Provider product page for 14-1249-82
Western blot
Immunocytochemistry
Flow cytometry
Other assay

Antibody data

Product number
14-1249-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD124 Monoclonal Antibody (X2/45-12), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The monoclonal antibody X2/45-12 reacts with human CD124, which is also known as the IL-4R alpha chain. CD124 can associate with the common gamma chain to form the type I IL-4R or with IL-13Ralpha1 to form the type II IL-4R. While both receptor types can bind IL-4, only the type II receptor can associate with IL-13. The type I IL-4 receptor is found on T, B, NK, mast cells, basophils, and macrophages. The type II IL-4 receptor is found on many non-hematopoietic cells and macrophages. CD124 contains an ITIM that associates with and activates STAT6. IL-4 is responsible for the generation of high-affinity IgE antibodies and driving the polarization of naive Th0 cells into Th2 cells. In mucosal barriers, the cytokine is involved in the recruitment of innate and adaptive immune effector cells and mucous overproduction. Finally, IL-4R also exists as a soluble form that serves primarily as a carrier protein for IL-4 that increases its half-life. The X2/45 antibody has been reported to have antagonist activity. Applications Reported: This X2/45-12 antibody has been reported for use in flow cytometric analysis, and western blotting. (Please use Functional Grade purified X2/45-12, Product # 16-1249-82, in functional assays). Applications Tested: This X2/45-12 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
X2/45-12
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C
IL4R protein structure - 14-1249-82 shown in red.