515002

antibody from BioLegend

Targeting: IL15 IL-15, MGC9721

ELISA (Data presented on provider website)

Antibody Data

Product number

515002

Provider

BioLegend

Proper citation

BioLegend Cat#515002, RRID:AB_2561319

Product name

Purified anti-human IL-15

Antibody type

Monoclonal

Antigen

E. coli - expressed, recombinant human IL-15

Description

Interleukin-15 (IL-15) was discovered in supernatant from simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, CTLL-2 (1). IL-15 is a regulatory cytokine that is produced by dendritic cells, epithelial cells, human stromal cell line (IMTLH), fibroblasts, and monocytes (2). It plays an important role in immune response and shares many functions with IL-2, for example, it stimulates the proliferation of activated T cells (1, 2), NK cells (3) and B cells, and it induces immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand (4). In addition, IL-15 promotes the development of dendritic cells (5), activates human neutrophils (6, 7) and induces the production of proinflammatory cytokines from macrophages (8). IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system. IL-15 binds to heterotrimeric receptors composed of IL-15Rα, IL-15Rβ, and IL-15Rγc. IL-15 shares with IL-2 the receptor chains β and γc. IL-15 is normally not secreted in soluble form but is held on the cell surface bound to a unique receptor, IL-15Rα, especially on dendritic cells. Cell-bound IL-15 then is presented in trans to T cells and NK cells and is recognized by the γc receptor on these cells; such recognition maintains cell survival and intermittent proliferation (9).

Reactivity

Human

Conjugate

Unconjugated

Antibody clone number

BH1509

Vial size

500 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT