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- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Immunohistochemistry [2]
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- Product number
- BAF447 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-15 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse IL-15 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human IL-15 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references The role of IL-15 in activating STAT5 and fine-tuning IL-17A production in CD4 T lymphocytes.
IFN-alpha enhances peptide vaccine-induced CD8+ T cell numbers, effector function, and antitumor activity.
IL-15Ralpha chaperones IL-15 to stable dendritic cell membrane complexes that activate NK cells via trans presentation.
Inhibition of tumor growth by NK1.1+ cells and CD8+ T cells activated by IL-15 through receptor beta/common gamma signaling in trans.
Inhibition of tumor growth by NK1.1+ cells and CD8+ T cells activated by IL-15 through receptor beta/common gamma signaling in trans.
IL-12 rapidly alters the functional profile of tumor-associated and tumor-infiltrating macrophages in vitro and in vivo.
IL-12 rapidly alters the functional profile of tumor-associated and tumor-infiltrating macrophages in vitro and in vivo.
Pandiyan P, Yang XP, Saravanamuthu SS, Zheng L, Ishihara S, O'Shea JJ, Lenardo MJ
Journal of immunology (Baltimore, Md. : 1950) 2012 Nov 1;189(9):4237-46
Journal of immunology (Baltimore, Md. : 1950) 2012 Nov 1;189(9):4237-46
IFN-alpha enhances peptide vaccine-induced CD8+ T cell numbers, effector function, and antitumor activity.
Sikora AG, Jaffarzad N, Hailemichael Y, Gelbard A, Stonier SW, Schluns KS, Frasca L, Lou Y, Liu C, Andersson HA, Hwu P, Overwijk WW
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7398-407
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7398-407
IL-15Ralpha chaperones IL-15 to stable dendritic cell membrane complexes that activate NK cells via trans presentation.
Mortier E, Woo T, Advincula R, Gozalo S, Ma A
The Journal of experimental medicine 2008 May 12;205(5):1213-25
The Journal of experimental medicine 2008 May 12;205(5):1213-25
Inhibition of tumor growth by NK1.1+ cells and CD8+ T cells activated by IL-15 through receptor beta/common gamma signaling in trans.
Rowley J, Monie A, Hung CF, Wu TC
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 15;181(12):8237-47
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 15;181(12):8237-47
Inhibition of tumor growth by NK1.1+ cells and CD8+ T cells activated by IL-15 through receptor beta/common gamma signaling in trans.
Rowley J, Monie A, Hung CF, Wu TC
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 15;181(12):8237-47
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 15;181(12):8237-47
IL-12 rapidly alters the functional profile of tumor-associated and tumor-infiltrating macrophages in vitro and in vivo.
Watkins SK, Egilmez NK, Suttles J, Stout RD
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1357-62
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1357-62
IL-12 rapidly alters the functional profile of tumor-associated and tumor-infiltrating macrophages in vitro and in vivo.
Watkins SK, Egilmez NK, Suttles J, Stout RD
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1357-62
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1357-62
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IL-15 in Mouse Intestine. IL-15 was detected in perfusion fixed frozen sections of mouse intestine using Mouse IL-15 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF447) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IL-15 in Mouse Intestine. IL-15 was detected in perfusion fixed frozen sections of mouse intestine using Mouse IL-15 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF447) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
