Antibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF315 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-15 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-15 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL-15 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references A First-in-Human Phase I Study of Subcutaneous Outpatient Recombinant Human IL15 (rhIL15) in Adults with Advanced Solid Tumors.
Discovery and characterization of a novel humanized anti-IL-15 antibody and its relevance for the treatment of refractory celiac disease and eosinophilic esophagitis.
IL-15 amplifies the pathogenic properties of CD4+CD28- T cells in multiple sclerosis.
Intramuscular delivery of heterodimeric IL-15 DNA in macaques produces systemic levels of bioactive cytokine inducing proliferation of NK and T cells.
TLR-9 and IL-15 Synergy Promotes the In Vitro Clonal Expansion of Chronic Lymphocytic Leukemia B Cells.
Enrichment and persistence of virus-specific CTL in the brain of simian immunodeficiency virus-infected monkeys is associated with a unique cytokine environment.
Enrichment and persistence of virus-specific CTL in the brain of simian immunodeficiency virus-infected monkeys is associated with a unique cytokine environment.
Cloning and expression of feline interleukin 15.
Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen.
NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen.
Miller JS, Morishima C, McNeel DG, Patel MR, Kohrt HEK, Thompson JA, Sondel PM, Wakelee HA, Disis ML, Kaiser JC, Cheever MA, Streicher H, Creekmore SP, Waldmann TA, Conlon KC
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Apr 1;24(7):1525-1535
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Apr 1;24(7):1525-1535
Discovery and characterization of a novel humanized anti-IL-15 antibody and its relevance for the treatment of refractory celiac disease and eosinophilic esophagitis.
Vicari AP, Schoepfer AM, Meresse B, Goffin L, Léger O, Josserand S, Guégan N, Yousefi S, Straumann A, Cerf-Bensussan N, Simon HU, Chvatchko Y
mAbs 2017 Aug Sep;9(6):927-944
mAbs 2017 Aug Sep;9(6):927-944
IL-15 amplifies the pathogenic properties of CD4+CD28- T cells in multiple sclerosis.
Broux B, Mizee MR, Vanheusden M, van der Pol S, van Horssen J, Van Wijmeersch B, Somers V, de Vries HE, Stinissen P, Hellings N
Journal of immunology (Baltimore, Md. : 1950) 2015 Mar 1;194(5):2099-109
Journal of immunology (Baltimore, Md. : 1950) 2015 Mar 1;194(5):2099-109
Intramuscular delivery of heterodimeric IL-15 DNA in macaques produces systemic levels of bioactive cytokine inducing proliferation of NK and T cells.
Bergamaschi C, Kulkarni V, Rosati M, Alicea C, Jalah R, Chen S, Bear J, Sardesai NY, Valentin A, Felber BK, Pavlakis GN
Gene therapy 2015 Jan;22(1):76-86
Gene therapy 2015 Jan;22(1):76-86
TLR-9 and IL-15 Synergy Promotes the In Vitro Clonal Expansion of Chronic Lymphocytic Leukemia B Cells.
Mongini PK, Gupta R, Boyle E, Nieto J, Lee H, Stein J, Bandovic J, Stankovic T, Barrientos J, Kolitz JE, Allen SL, Rai K, Chu CC, Chiorazzi N
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 1;195(3):901-23
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 1;195(3):901-23
Enrichment and persistence of virus-specific CTL in the brain of simian immunodeficiency virus-infected monkeys is associated with a unique cytokine environment.
Marcondes MC, Burdo TH, Sopper S, Huitron-Resendiz S, Lanigan C, Watry D, Flynn C, Zandonatti M, Fox HS
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5812-9
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5812-9
Enrichment and persistence of virus-specific CTL in the brain of simian immunodeficiency virus-infected monkeys is associated with a unique cytokine environment.
Marcondes MC, Burdo TH, Sopper S, Huitron-Resendiz S, Lanigan C, Watry D, Flynn C, Zandonatti M, Fox HS
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5812-9
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5812-9
Cloning and expression of feline interleukin 15.
Dean GA, Barger A, Lavoy A
Cytokine 2005 Jan 21;29(2):77-83
Cytokine 2005 Jan 21;29(2):77-83
Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 15;174(4):2376-86
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 15;174(4):2376-86
Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 15;174(4):2376-86
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 15;174(4):2376-86
NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen.
Vankayalapati R, Klucar P, Wizel B, Weis SE, Samten B, Safi H, Shams H, Barnes PF
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):130-7
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):130-7
NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen.
Vankayalapati R, Klucar P, Wizel B, Weis SE, Samten B, Safi H, Shams H, Barnes PF
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):130-7
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):130-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IL-15 in Human Tonsil. IL-15 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF315) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-15 and Neutralization by Human IL-15 Antibody. Recombinant Human IL-15 (Catalog # 247-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-15 (10 ) is neutralized (green line) by increasing concen-trations of Goat Anti-Human IL-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF315). The ND50 is typically 0.5-1.5 µg/mL.