MAB647-100

antibody from Novus Biologicals

Targeting: IL15 IL-15, MGC9721

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB647-100

Provider

Novus Biologicals

Product name

Mouse Monoclonal IL-15 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human IL-15 in direct ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human (rh) IL-1 alpha , -1 beta , -2, -3, -4, -5, -6, -7, -8, -9, rmIL-1 alpha , -1 beta , -3, -4, -5, -6, -7, -9, or -13 is observed.

Reactivity

Human, Simian

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-15 and Neutralization by Human IL-15 Antibody. Recombinant Human IL-15 (Catalog # 247-ILB) induces proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-15 (3 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL-15 Monoclonal Antibody (Catalog # MAB647). The ND₅₀ is typically 4-20 ng/mL.