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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Blocking/Neutralizing [1]
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- Product number
- MAB647-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Primate IL-15 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-15 in direct ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human (rh) IL-1 alpha , -1 beta , -2, -3, -4, -5, -6, -7, -8, -9, rmIL-1 alpha , -1 beta , -3, -4, -5, -6, -7, -9, or -13 is observed.
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P40933- Isotype
- IgG
- Antibody clone number
- 34505
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling.
IL-15 controls T cell functions through its influence on CD30 and OX40 antigens in Celiac Disease.
Epstein-Barr virus coinfection in children boosts cytomegalovirus-induced differentiation of natural killer cells.
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Histological characteristics and altered expression of interleukins (IL) IL-13 and IL-15 in endometria of levonorgestrel users with different uterine bleeding patterns.
Growth and apoptosis of human natural killer cell neoplasms: role of interleukin-2/15 signaling.
NK cell activation by dendritic cells (DCs) requires the formation of a synapse leading to IL-12 polarization in DCs.
Clinical improvement in chronic plaque-type psoriasis lesions after narrow-band UVB therapy is accompanied by a decrease in the expression of IFN-gamma inducers -- IL-12, IL-18 and IL-23.
Jiang J, Chen X, An H, Yang B, Zhang F, Cheng X
Scientific reports 2016 Sep 2;6:32320
Scientific reports 2016 Sep 2;6:32320
IL-15 controls T cell functions through its influence on CD30 and OX40 antigens in Celiac Disease.
Periolo N, Guillén L, Arruvito ML, Alegre NS, Niveloni SI, Hwang JH, Bai JC, Cherñavsky AC
Cytokine 2014 May;67(1):44-51
Cytokine 2014 May;67(1):44-51
Epstein-Barr virus coinfection in children boosts cytomegalovirus-induced differentiation of natural killer cells.
Saghafian-Hedengren S, Sohlberg E, Theorell J, Carvalho-Queiroz C, Nagy N, Persson JO, Nilsson C, Bryceson YT, Sverremark-Ekström E
Journal of virology 2013 Dec;87(24):13446-55
Journal of virology 2013 Dec;87(24):13446-55
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS research and human retroviruses 2008 Mar;24(3):453-62
AIDS research and human retroviruses 2008 Mar;24(3):453-62
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood 2007 May 15;109(10):4272-9
Blood 2007 May 15;109(10):4272-9
Histological characteristics and altered expression of interleukins (IL) IL-13 and IL-15 in endometria of levonorgestrel users with different uterine bleeding patterns.
Rhoton-Vlasak A, Chegini N, Hardt N, Williams RS
Fertility and sterility 2005 Mar;83(3):659-65
Fertility and sterility 2005 Mar;83(3):659-65
Growth and apoptosis of human natural killer cell neoplasms: role of interleukin-2/15 signaling.
Yamasaki S, Maeda M, Ohshima K, Kikuchi M, Otsuka T, Harada M
Leukemia research 2004 Oct;28(10):1023-31
Leukemia research 2004 Oct;28(10):1023-31
NK cell activation by dendritic cells (DCs) requires the formation of a synapse leading to IL-12 polarization in DCs.
Borg C, Jalil A, Laderach D, Maruyama K, Wakasugi H, Charrier S, Ryffel B, Cambi A, Figdor C, Vainchenker W, Galy A, Caignard A, Zitvogel L
Blood 2004 Nov 15;104(10):3267-75
Blood 2004 Nov 15;104(10):3267-75
Clinical improvement in chronic plaque-type psoriasis lesions after narrow-band UVB therapy is accompanied by a decrease in the expression of IFN-gamma inducers -- IL-12, IL-18 and IL-23.
Piskin G, Tursen U, Sylva-Steenland RM, Bos JD, Teunissen MB
Experimental dermatology 2004 Dec;13(12):764-72
Experimental dermatology 2004 Dec;13(12):764-72
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-15 and Neutralization by Human IL-15 Antibody. Recombinant Human IL-15 (Catalog # 247-ILB) induces proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-15 (3 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL-15 Monoclonal Antibody (Catalog # MAB647). The ND50 is typically 4-20 ng/mL.
