Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF447 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-15 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse IL-15 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human IL-15 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P48346- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references TCR-pMHC encounter differentially regulates transcriptomes of tissue-resident CD8 T cells.
Transcription factor KLF2 regulates homeostatic NK cell proliferation and survival.
ESAT-6-dependent cytosolic pattern recognition drives noncognate tuberculosis control in vivo.
Combination of TACI-IgG and anti-IL-15 treats murine lupus by reducing mature and memory B cells.
Blockade of IL-15 activity inhibits microglial activation through the NFkappaB, p38, and ERK1/2 pathways, reducing cytokine and chemokine release.
IL-15Ralpha chaperones IL-15 to stable dendritic cell membrane complexes that activate NK cells via trans presentation.
A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex.
Yoshizawa A, Bi K, Keskin DB, Zhang G, Reinhold B, Reinherz EL
European journal of immunology 2018 Jan;48(1):128-150
European journal of immunology 2018 Jan;48(1):128-150
Transcription factor KLF2 regulates homeostatic NK cell proliferation and survival.
Rabacal W, Pabbisetty SK, Hoek KL, Cendron D, Guo Y, Maseda D, Sebzda E
Proceedings of the National Academy of Sciences of the United States of America 2016 May 10;113(19):5370-5
Proceedings of the National Academy of Sciences of the United States of America 2016 May 10;113(19):5370-5
ESAT-6-dependent cytosolic pattern recognition drives noncognate tuberculosis control in vivo.
Kupz A, Zedler U, Stäber M, Perdomo C, Dorhoi A, Brosch R, Kaufmann SH
The Journal of clinical investigation 2016 Jun 1;126(6):2109-22
The Journal of clinical investigation 2016 Jun 1;126(6):2109-22
Combination of TACI-IgG and anti-IL-15 treats murine lupus by reducing mature and memory B cells.
Ma N, Xiao H, Marrero B, Xing C, Wang X, Zheng M, Han G, Chen G, Hou C, Shen B, Li Y, Wang R, Jiang Z
Cellular immunology 2014 May-Jun;289(1-2):140-4
Cellular immunology 2014 May-Jun;289(1-2):140-4
Blockade of IL-15 activity inhibits microglial activation through the NFkappaB, p38, and ERK1/2 pathways, reducing cytokine and chemokine release.
Gomez-Nicola D, Valle-Argos B, Nieto-Sampedro M
Glia 2010 Feb;58(3):264-76
Glia 2010 Feb;58(3):264-76
IL-15Ralpha chaperones IL-15 to stable dendritic cell membrane complexes that activate NK cells via trans presentation.
Mortier E, Woo T, Advincula R, Gozalo S, Ma A
The Journal of experimental medicine 2008 May 12;205(5):1213-25
The Journal of experimental medicine 2008 May 12;205(5):1213-25
A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex.
Rafei M, Wu JH, Annabi B, Lejeune L, François M, Galipeau J
Blood 2007 Mar 1;109(5):2234-42
Blood 2007 Mar 1;109(5):2234-42
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-15 in Mouse Intestine. IL-15 was detected in immersion fixed frozen sections of mouse intestine (Peyer patch) using Goat Anti-Mouse IL-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF447) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes in villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-15 and Neutralization by Mouse IL-15 Antibody. Recombinant Mouse IL-15 (Catalog # 447-ML) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-15 (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF447). The ND50 is typically 0.4-2.4 µg/mL.
