16-0157-82

antibody from Invitrogen Antibodies
Targeting: IL15 IL-15, MGC9721
Provider product page for 16-0157-82
Blocking/Neutralizing
Functional assay
Other assay

Antibody data

Product number
16-0157-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
IL-15 Monoclonal Antibody (ct2nu), Functional Grade, eBioscience™
Antibody type
Monoclonal
Antigen
Recombinant full-length protein
Description
Description: The ct2nu monoclonal antibody reacts with human IL-15 and neutralizes its bioactivity. IL-15, or IL-T, is a 14 kDa proinflammatory cytokine and member of the IL-2 family. It plays a role in the development and maturation of macrophages and dendritic cells, and is essential to the proliferation and survival of NK cells and CD8^+ T cells. IL-15 is expressed by antigen-presenting cells, epithelial cells, and stromal cells. IL-15 activates the Jak/STAT pathway through its heterotrimeric receptor. Two subunits of this receptor, the beta and gamma chains, are shared with the rest of the family, while the alpha subunit is unique to IL-15. In mice, the alpha chain is required to chaperone IL-15 from the ER to secretion. This chaperoning is not necessary in humans, and free IL-15 is detectable in human biological samples. However, the receptor-cytokine complex has been indentified in humans and demonstrates agonistic activity several-fold higher than IL-15 alone. Applications Reported: The monoclonal antibody ct2nu reacts with and inhibits the bioactivity of human IL-15. Applications Tested: The ND50 of this antibody, as measured by inhibition of proliferation in CTLL-2 cells, is 1-10 ng/mL in the presence of 0.5 ng/mL recombinant human IL-15. Neutralization dose will vary based on cell type, cytokine concentration, and assay method. This antibody should be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
ct2nu
Vial size
100 µg
Concentration
1 mg/mL
Storage
4°C
IL15 protein structure - 16-0157-82 shown in red.