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- Antibody Data
- Antigen structure
- References [4]
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- ELISA [1]
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- Product number
- 500-P15-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-15 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-15. Anti-Human IL-15-specific antibody was purified by affinity chromatography employing an immobilized Human IL-15 matrix. Sandwich ELISA: To detect Human IL-15 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-15 (500-P15Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-15. Western Blot: To detect Human IL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-15 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references CCL18/PARC stimulates hematopoiesis in long-term bone marrow cultures indirectly through its effect on monocytes.
Interleukin-7 and interleukin-15 regulate the expression of the bcl-2 and c-myb genes in cutaneous T-cell lymphoma cells.
Targeting the IL-15 receptor with an antagonist IL-15 mutant/Fc gamma2a protein blocks delayed-type hypersensitivity.
Interleukin-15 is an autocrine/paracrine viability factor for cutaneous T-cell lymphoma cells.
Wimmer A, Khaldoyanidi SK, Judex M, Serobyan N, Discipio RG, Schraufstatter IU
Blood 2006 Dec 1;108(12):3722-9
Blood 2006 Dec 1;108(12):3722-9
Interleukin-7 and interleukin-15 regulate the expression of the bcl-2 and c-myb genes in cutaneous T-cell lymphoma cells.
Qin JZ, Zhang CL, Kamarashev J, Dummer R, Burg G, Döbbeling U
Blood 2001 Nov 1;98(9):2778-83
Blood 2001 Nov 1;98(9):2778-83
Targeting the IL-15 receptor with an antagonist IL-15 mutant/Fc gamma2a protein blocks delayed-type hypersensitivity.
Kim YS, Maslinski W, Zheng XX, Stevens AC, Li XC, Tesch GH, Kelley VR, Strom TB
Journal of immunology (Baltimore, Md. : 1950) 1998 Jun 15;160(12):5742-8
Journal of immunology (Baltimore, Md. : 1950) 1998 Jun 15;160(12):5742-8
Interleukin-15 is an autocrine/paracrine viability factor for cutaneous T-cell lymphoma cells.
Döbbeling U, Dummer R, Laine E, Potoczna N, Qin JZ, Burg G
Blood 1998 Jul 1;92(1):252-8
Blood 1998 Jul 1;92(1):252-8
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA: To detect Human IL-15 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of IL-15 Polyclonal Antibody (Product # 500-P15-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech IL-15 Polyclonal Antibody, Biotin (Product # 500-P15BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-15.
