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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Flow cytometry [1]
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- Product number
- IC2471F - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-15 Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-15 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2, recombinant mouse IL-15, or rhIL-21 is observed.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
P40933- Isotype
- IgG
- Antibody clone number
- 34559
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Phenotypic and functional characterization of NK cells in human immune response against the dimorphic fungus Paracoccidioides brasiliensis.
Herpes simplex virus-1 up-regulates IL-15 gene expression in monocytic cells through the activation of protein tyrosine kinase and PKC zeta/lambda signaling pathways.
Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs.
IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
Longhi LN, da Silva RM, Fornazim MC, Spago MC, de Oliveira RT, Nowill AE, Blotta MH, Mamoni RL
Journal of immunology (Baltimore, Md. : 1950) 2012 Jul 15;189(2):935-45
Journal of immunology (Baltimore, Md. : 1950) 2012 Jul 15;189(2):935-45
Herpes simplex virus-1 up-regulates IL-15 gene expression in monocytic cells through the activation of protein tyrosine kinase and PKC zeta/lambda signaling pathways.
Ahmad R, Ennaciri J, Cordeiro P, El Bassam S, Menezes J
Journal of molecular biology 2007 Mar 16;367(1):25-35
Journal of molecular biology 2007 Mar 16;367(1):25-35
Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs.
Ferlazzo G, Pack M, Thomas D, Paludan C, Schmid D, Strowig T, Bougras G, Muller WA, Moretta L, Münz C
Proceedings of the National Academy of Sciences of the United States of America 2004 Nov 23;101(47):16606-11
Proceedings of the National Academy of Sciences of the United States of America 2004 Nov 23;101(47):16606-11
IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
Miranda-Carús ME, Balsa A, Benito-Miguel M, Pérez de Ayala C, Martín-Mola E
Journal of immunology (Baltimore, Md. : 1950) 2004 Jul 15;173(2):1463-76
Journal of immunology (Baltimore, Md. : 1950) 2004 Jul 15;173(2):1463-76
IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
Miranda-Carús ME, Balsa A, Benito-Miguel M, Pérez de Ayala C, Martín-Mola E
Journal of immunology (Baltimore, Md. : 1950) 2004 Jul 15;173(2):1463-76
Journal of immunology (Baltimore, Md. : 1950) 2004 Jul 15;173(2):1463-76
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of IL-15 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with LPS were stained with Mouse Anti-Human IL-15 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC2471F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
