IC2471P

antibody from R&D Systems

Targeting: IL15 IL-15, MGC9721

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC2471P

Provider

R&D Systems

Product name

Human IL-15 PE-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human IL-15 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2, recombinant mouse IL-15, or rhIL-21 is observed.

Reactivity

Human

Host

Mouse

Antigen sequence

P40933


Isotype

IgG

Antibody clone number

34559

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

References

Submitted references

Impaired TGF-β signaling in patients with active systemic lupus erythematosus is associated with an overexpression of IL-22.

Rekik R, Smiti Khanfir M, Larbi T, Zamali I, Beldi-Ferchiou A, Kammoun O, Marzouki S, Hamzaoui S, Mrad S, Barbouche MR, Houman MH, Ben Ahmed M
Cytokine 2018 Aug;108:182-189

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Dendritic Cell-Derived IL-32α: A Novel Inhibitory Cytokine of NK Cell Function.

Gorvel L, Korenfeld D, Tung T, Klechevsky E
Journal of immunology (Baltimore, Md. : 1950) 2017 Aug 15;199(4):1290-1300

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Interleukin-15 Dendritic Cells Harness NK Cell Cytotoxic Effector Function in a Contact- and IL-15-Dependent Manner.

Anguille S, Van Acker HH, Van den Bergh J, Willemen Y, Goossens H, Van Tendeloo VF, Smits EL, Berneman ZN, Lion E
PloS one 2015;10(5):e0123340

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Interleukin-15 enhances cellular proliferation and upregulates CNS homing molecules in pre-B acute lymphoblastic leukemia.

Williams MT, Yousafzai Y, Cox C, Blair A, Carmody R, Sai S, Chapman KE, McAndrew R, Thomas A, Spence A, Gibson B, Graham GJ, Halsey C
Blood 2014 May 15;123(20):3116-27

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Qualitatively different T cell phenotypic responses to IL-2 versus IL-15 are unified by identical dependences on receptor signal strength and duration.

Arneja A, Johnson H, Gabrovsek L, Lauffenburger DA, White FM
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 1;192(1):123-35

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CD8+ T cells are activated in an antigen-independent manner in HIV-infected individuals.

Bastidas S, Graw F, Smith MZ, Kuster H, Günthard HF, Oxenius A
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 15;192(4):1732-44

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Survival and migration of human dendritic cells are regulated by an IFN-alpha-inducible Axl/Gas6 pathway.

Scutera S, Fraone T, Musso T, Cappello P, Rossi S, Pierobon D, Orinska Z, Paus R, Bulfone-Paus S, Giovarelli M
Journal of immunology (Baltimore, Md. : 1950) 2009 Sep 1;183(5):3004-13

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A novel small molecule inhibitor of signal transducers and activators of transcription 3 reverses immune tolerance in malignant glioma patients.

Hussain SF, Kong LY, Jordan J, Conrad C, Madden T, Fokt I, Priebe W, Heimberger AB
Cancer research 2007 Oct 15;67(20):9630-6

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Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs.

Ferlazzo G, Pack M, Thomas D, Paludan C, Schmid D, Strowig T, Bougras G, Muller WA, Moretta L, Münz C
Proceedings of the National Academy of Sciences of the United States of America 2004 Nov 23;101(47):16606-11

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of IL-15 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with LPS were stained with Mouse Anti-Human IL-15 PE-conjugated Monoclonal Antibody (Catalog # IC2471P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.