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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3469 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3469, RRID:AB_1576388
- Product name
- IRAK1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of IRAK1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Pellino 1 is required for interleukin-1 (IL-1)-mediated signaling through its interaction with the IL-1 receptor-associated kinase 4 (IRAK4)-IRAK-tumor necrosis factor receptor-associated factor 6 (TRAF6) complex.
Pellino3, a novel member of the Pellino protein family, promotes activation of c-Jun and Elk-1 and may act as a scaffolding protein.
IRAK: a kinase associated with the interleukin-1 receptor.
Jiang Z, Johnson HJ, Nie H, Qin J, Bird TA, Li X
The Journal of biological chemistry 2003 Mar 28;278(13):10952-6
The Journal of biological chemistry 2003 Mar 28;278(13):10952-6
Pellino3, a novel member of the Pellino protein family, promotes activation of c-Jun and Elk-1 and may act as a scaffolding protein.
Jensen LE, Whitehead AS
Journal of immunology (Baltimore, Md. : 1950) 2003 Aug 1;171(3):1500-6
Journal of immunology (Baltimore, Md. : 1950) 2003 Aug 1;171(3):1500-6
IRAK: a kinase associated with the interleukin-1 receptor.
Cao Z, Henzel WJ, Gao X
Science (New York, N.Y.) 1996 Feb 23;271(5252):1128-31
Science (New York, N.Y.) 1996 Feb 23;271(5252):1128-31
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of IRAK1 polyclonal antibody (Cat # PAB3469) in Jurkat cell lysate. IRAK1 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with IRAK1 polyclonal antibody (Cat # PAB3469) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
