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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PAB10249 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10249, RRID:AB_1676578
- Product name
- IRAK1 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to C-terminus of human IRAK1.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100 μg
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin 1 receptor accessory protein.
Developmental and tissue-specific expression of mouse pelle-like protein kinase.
IRAK: a kinase associated with the interleukin-1 receptor.
Huang J, Gao X, Li S, Cao Z
Proceedings of the National Academy of Sciences of the United States of America 1997 Nov 25;94(24):12829-32
Proceedings of the National Academy of Sciences of the United States of America 1997 Nov 25;94(24):12829-32
Developmental and tissue-specific expression of mouse pelle-like protein kinase.
Trofimova M, Sprenkle AB, Green M, Sturgill TW, Goebl MG, Harrington MA
The Journal of biological chemistry 1996 Jul 26;271(30):17609-12
The Journal of biological chemistry 1996 Jul 26;271(30):17609-12
IRAK: a kinase associated with the interleukin-1 receptor.
Cao Z, Henzel WJ, Gao X
Science (New York, N.Y.) 1996 Feb 23;271(5252):1128-31
Science (New York, N.Y.) 1996 Feb 23;271(5252):1128-31
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using IRAK1 polyclonal antibody (Cat # PAB10249) shows detection of IRAK1 in THP-1 whole cell lysate (THP) or HeLa whole cell lysate (HL). The membrane was probed with the primary antibody diluted to 1 : 2,000.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunocytochemistry using IRAK1 polyclonal antibody (Cat # PAB10249) shows staining of IRAK1 in HeLa cells.The cells were fixed with paraformaldehyde and reacted with the primary antibody diluted to 1 : 100.
- Validation comment
- Immunocytochemistry
