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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- 710015 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRAK1 Recombinant Polyclonal Antibody (19HCLC)
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 19HCLC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IRAK1 was performed by loading 20 µg of HEK-293 (lane1), LNCaP (lane2), NIH/3T3 (lane3), HeLa (lane4), HT-29 (lane5), MCF7 (lane6) and K562 (lane7) cell lysate using Novex® NuPAGE®4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. IRAK1 was detected at ~ 80 kDa using IRAK1 Recombinant Rabbit Polyclonal Antibody (Product # 710015) at 1:1000 dilution in 5 % skim milk at 4ºC overnight on a rocking platform. Goat anti-Rabbit IgG - HRP Secondary Antibody (Product # G-21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IRAK1 was performed by loading 20 µg of HEK-293 (lane1), LNCaP (lane2), NIH/3T3 (lane3), HeLa (lane4), HT-29 (lane5), MCF7 (lane6) and K562 (lane7) cell lysate using Novex® NuPAGE®4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. IRAK1 was detected at ~ 80 kDa using IRAK1 Recombinant Rabbit Polyclonal Antibody (Product # 710015) at 1:1000 dilution in 5 % skim milk at 4ºC overnight on a rocking platform. Goat anti-Rabbit IgG - HRP Secondary Antibody (Product # G-21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IRAK-1 in HEK293 cell lysate (30 µg/lane) using an IRAK-1 Recombinant Rabbit Polyclonal Antibody (Product # 710015) at a dilution of 5 µg/mL. NBT/BCIP was used as the substrate (Product # WB7105).
