Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Other assay [6]
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- Product number
- PA5-17490 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRAK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 26.4 µg/mL
- Storage
- -20°C
Submitted references Viperin interacts with the kinase IRAK1 and the E3 ubiquitin ligase TRAF6, coupling innate immune signaling to antiviral ribonucleotide synthesis.
Human Cytomegalovirus miR-UL112-3p Targets TLR2 and Modulates the TLR2/IRAK1/NFκB Signaling Pathway.
Dumbrepatil AB, Ghosh S, Zegalia KA, Malec PA, Hoff JD, Kennedy RT, Marsh ENG
The Journal of biological chemistry 2019 Apr 26;294(17):6888-6898
The Journal of biological chemistry 2019 Apr 26;294(17):6888-6898
Human Cytomegalovirus miR-UL112-3p Targets TLR2 and Modulates the TLR2/IRAK1/NFκB Signaling Pathway.
Landais I, Pelton C, Streblow D, DeFilippis V, McWeeney S, Nelson JA
PLoS pathogens 2015 May;11(5):e1004881
PLoS pathogens 2015 May;11(5):e1004881
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IRAK1 in extracts from THP-1 cells, differentiated with TPA (80 nM for 24h) and treated with 1 µg/mL LPS, using IRAK1 polyclonal antibody (Product # PA5-17490).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IRAK1 in extracts from HeLa and 293 cells using IRAK1 polyclonal antibody (Product # PA5-17490).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Interleukin-1 receptor-associated kinase 1 was achieved by transfecting K-562 with Interleukin-1 receptor-associated kinase 1 specific siRNAs (Silencer® select Product # s322, s324). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Interleukin-1 receptor-associated kinase 1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with IRAK1 Polyclonal Antibody (Product # PA5-17490, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Interleukin-1 receptor-associated kinase 1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), MCF7 (Lane 3), HT-1080 (Lane 4), COLO 205 (Lane 5), MDA-MB-231 (Lane 6), COS-7 (Lane 7) and THP-1 (Lane 8). The blot was probed with Anti-IRAK1 Polyclonal Antibody (Product # PA5-17490, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). An 80kDa band corresponding to IRAK1 was observed across all the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-IRAK1 Polyclonal Antibody (Product # PA5-17490) and a 80 kDa band corresponding to Interleukin-1 receptor-associated kinase 1 was observed across tested cell lines. Whole cell extracts (40 µg lysate) of THP-1 (Lane 1), Jurkat (Lane 2), K-562 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of IRAK1 in HT-1080 cells using an IRAK1 polyclonal antibody (Product # PA5-17490) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
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- Invitrogen Antibodies (provider)
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- Invitrogen Antibodies (provider)
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- Invitrogen Antibodies (provider)
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- Invitrogen Antibodies (provider)
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- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Fig 5 miR-UL112-3p inhibits TLR2-dependent activation of IRAK1. (A) Schematic of the upper section of the TLR2/NFkappaB pathway with known factors and post-translational modifications of IRAK4 and IRAK1 upon stimulation with TLR2 agonists. Grey ellipse, agonist; P, phosphorylation; K63, Lys63 poly-ubiquitin chain. (B) IRAK1 undergoes post-translational modifications upon stimulation with a TLR2/TLR1 agonist (PAM3CSK4, 100ng/ml) but not with a TLR4 agonist (LPS). TPA-differentiated THP-1 cells were treated as described in panel A. Unmodified and post-translationally modified forms of IRAK1 were detected by IB. (C) miR-UL112-3p inhibits FSL-1 induced IRAK1 post-translational modifications. TLR2, beta-actin and IRAK1 unmodified and modified forms were detected by IB. Numbers below the IRAK1 blot represent quantification (in relative units) of the unmodified protein signal.