Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1387 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal JNK1/2/3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse, and rat p46 and p54 JNK in Western blots. Detects recombinant human (rh) JNK1, rhJNK2, and rhJNK3.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Dysregulation of the splicing machinery is directly associated to aggressiveness of prostate cancer.
Role of the MAPK/cJun NH(2)-terminal kinase signaling pathway in starvation-induced autophagy.
JNK Promotes Epithelial Cell Anoikis by Transcriptional and Post-translational Regulation of BH3-Only Proteins.
The c-Jun N-terminal kinase prevents oxidative stress induced by UV and thermal stresses in corals and human cells.
White-to-brown metabolic conversion of human adipocytes by JAK inhibition.
Oxidized {alpha}1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
Type I IFNs play a role in early resistance, but subsequent susceptibility, to the African trypanosomes.
Type I IFNs play a role in early resistance, but subsequent susceptibility, to the African trypanosomes.
Distinct role of c-Jun N-terminal kinase isoforms in human neutrophil apoptosis regulated by tumor necrosis factor-alpha and granulocyte-macrophage colony-stimulating factor.
Jiménez-Vacas JM, Herrero-Aguayo V, Montero-Hidalgo AJ, Gómez-Gómez E, Fuentes-Fayos AC, León-González AJ, Sáez-Martínez P, Alors-Pérez E, Pedraza-Arévalo S, González-Serrano T, Reyes O, Martínez-López A, Sánchez-Sánchez R, Ventura S, Yubero-Serrano EM, Requena-Tapia MJ, Castaño JP, Gahete MD, Luque RM
EBioMedicine 2020 Jan;51:102547
EBioMedicine 2020 Jan;51:102547
Role of the MAPK/cJun NH(2)-terminal kinase signaling pathway in starvation-induced autophagy.
Barutcu SA, Girnius N, Vernia S, Davis RJ
Autophagy 2018;14(9):1586-1595
Autophagy 2018;14(9):1586-1595
JNK Promotes Epithelial Cell Anoikis by Transcriptional and Post-translational Regulation of BH3-Only Proteins.
Girnius N, Davis RJ
Cell reports 2017 Nov 14;21(7):1910-1921
Cell reports 2017 Nov 14;21(7):1910-1921
The c-Jun N-terminal kinase prevents oxidative stress induced by UV and thermal stresses in corals and human cells.
Courtial L, Picco V, Grover R, Cormerais Y, Rottier C, Labbe A, Pagès G, Ferrier-Pagès C
Scientific reports 2017 Apr 4;7:45713
Scientific reports 2017 Apr 4;7:45713
White-to-brown metabolic conversion of human adipocytes by JAK inhibition.
Moisan A, Lee YK, Zhang JD, Hudak CS, Meyer CA, Prummer M, Zoffmann S, Truong HH, Ebeling M, Kiialainen A, Gérard R, Xia F, Schinzel RT, Amrein KE, Cowan CA
Nature cell biology 2015 Jan;17(1):57-67
Nature cell biology 2015 Jan;17(1):57-67
Oxidized {alpha}1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R
American journal of physiology. Lung cellular and molecular physiology 2009 Aug;297(2):L388-400
American journal of physiology. Lung cellular and molecular physiology 2009 Aug;297(2):L388-400
Type I IFNs play a role in early resistance, but subsequent susceptibility, to the African trypanosomes.
Lopez R, Demick KP, Mansfield JM, Paulnock DM
Journal of immunology (Baltimore, Md. : 1950) 2008 Oct 1;181(7):4908-17
Journal of immunology (Baltimore, Md. : 1950) 2008 Oct 1;181(7):4908-17
Type I IFNs play a role in early resistance, but subsequent susceptibility, to the African trypanosomes.
Lopez R, Demick KP, Mansfield JM, Paulnock DM
Journal of immunology (Baltimore, Md. : 1950) 2008 Oct 1;181(7):4908-17
Journal of immunology (Baltimore, Md. : 1950) 2008 Oct 1;181(7):4908-17
Distinct role of c-Jun N-terminal kinase isoforms in human neutrophil apoptosis regulated by tumor necrosis factor-alpha and granulocyte-macrophage colony-stimulating factor.
Kato T, Noma H, Kitagawa M, Takahashi T, Oshitani N, Kitagawa S
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2008 Apr;28(4):235-43
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2008 Apr;28(4):235-43
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat JNK by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U937 human histiocytic lymphoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands for JNK were detected at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of recombinant JNK family members by Western Blot. Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- JNK in Rat Kidney. JNK was detected in perfusion fixed frozen sections of rat kidney using Rabbit Anti-Human/Mouse/Rat JNK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- JNK in Human Prostate Cancer Tissue. JNK was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Rabbit Anti-Human/Mouse/Rat JNK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.