Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AM00082PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM00082PU-N, RRID:AB_1007210
- Product name
- anti MAPK8 / JNK1 (pT-G/P-pY Motif) (incl. pos. control)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide conjugated to KLH.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9H8
- Vial size
- 0.1 mg
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Immunoblot Analysis Jurkat cells were cultured under serum-free conditions for 48h and subsequently were either unstimulated (lane 1) or stimulated with 300mM sorbit (lane 2), 1mM arsenite (lane 3) or 1mM pervanadate (lane 4).The cell lysates were separated by SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab SAPK1/2-9H8 at 0.5µg/ml for 1h at 15-22°C and developed by ECL .