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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
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- Product number
- MAB1387 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat JNK Pan Specific Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat p46 and p54 JNK in Western blots. Detects recombinant JNK1, JNK2, and JNK3.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P45983- Isotype
- IgG
- Antibody clone number
- 252355
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Uncaria tomentosa improves insulin sensitivity and inflammation in experimental NAFLD.
Common dysregulation of Wnt/Frizzled receptor elements in human hepatocellular carcinoma.
Araujo LCC, Feitosa KB, Murata GM, Furigo IC, Teixeira SA, Lucena CF, Ribeiro LM, Muscará MN, Costa SKP, Donato J Jr, Bordin S, Curi R, Carvalho CRO
Scientific reports 2018 Jul 20;8(1):11013
Scientific reports 2018 Jul 20;8(1):11013
Common dysregulation of Wnt/Frizzled receptor elements in human hepatocellular carcinoma.
Bengochea A, de Souza MM, Lefrançois L, Le Roux E, Galy O, Chemin I, Kim M, Wands JR, Trepo C, Hainaut P, Scoazec JY, Vitvitski L, Merle P
British journal of cancer 2008 Jul 8;99(1):143-50
British journal of cancer 2008 Jul 8;99(1):143-50
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse JNK by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat JNK Pan Specific Monoclonal Antibody (Catalog # MAB1387) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands for JNK were detected at approximately 46 kDa (p46 JNK) and 54 kDa (p54 JNK) (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human JNK by Western Blot. Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat JNK Pan Specific Monoclonal Antibody (Catalog # MAB1387) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
