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Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Proximity ligation assay [1]
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- Product number
- H00005599-M08 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005599-M08, RRID:AB_1112059
- Product name
- MAPK8 monoclonal antibody (M08), clone 1E2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant MAPK8.
- Antigen sequence
HPYINVWYDPSEAEAPPPKIPDKQLDEREHTIEEW
KELIYKEVMDLEERTKNGVIRGQPSPLGAAVINGS
QHPSSSSSVNDVSSMSTDPTLASDTDSSLEAAAGP
LGCCR- Isotype
- IgG
- Antibody clone number
- 1E2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between CDC42 and MAPK8. HeLa cells were stained with anti-CDC42 rabbit purified polyclonal 1:1200 and anti-MAPK8 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
