H00005599-D01P

antibody from Abnova Corporation

Targeting: MAPK8 JNK, JNK1, PRKM8, SAPK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005599-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005599-D01P, RRID:AB_1577755

Product name

MAPK8 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human MAPK8 protein.

Antigen sequence

MSRSKRDNNFYSVEIGDSTFTVLKRYQNLKPIGSG
AQGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRA
YRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVY
IVMELMDANLCQVIQMELDHERMSYLLYQMLCGIK
HLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLART
AGTSFMMTPYVVTRYYRAPEVILGMGYKENVDLWS
VGCIMGEMVCHKILFPGRDYIDQWNKVIEQLGTPC
PEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLF
PADSEHNKLKASQARDLLSKMLVIDASKRISVDEA
LQHPYINVWYDPSEAEAPPPKIPDKQLDEREHTIE
EWKELIYKEVMDLEERTKNGVIRGQPSPLGAAVIN
GSQHPSSSSSVNDVSSMSTDPTLASDTDSSLEAAA
GPLGCCR


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MAPK8 expression in transfected 293T cell line (H00005599-T01) by MAPK8 MaxPab polyclonal antibody.Lane 1: MAPK8 transfected lysate(48.30 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

MAPK8 MaxPab rabbit polyclonal antibody. Western Blot analysis of MAPK8 expression in HeLa.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to MAPK8 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between MAPK8 and FASLG. HeLa cells were stained with anti-MAPK8 rabbit purified polyclonal 1:1200 and anti-FASLG mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)