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- Western blot [2]
- ELISA [1]
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- Product number
- H00005599-M11 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005599-M11, RRID:AB_1146923
- Product name
- Mouse Monoclonal JNK1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. MAPK8 - mitogen-activated protein kinase 8 (2F11)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Phosphorylation of Glutathione S-Transferase P1 (GSTP1) by Epidermal Growth Factor Receptor (EGFR) Promotes Formation of the GSTP1-c-Jun N-terminal kinase (JNK) Complex and Suppresses JNK Downstream Signaling and Apoptosis in Brain Tumor Cells.
Okamura T, Antoun G, Keir ST, Friedman H, Bigner DD, Ali-Osman F
The Journal of biological chemistry 2015 Dec 25;290(52):30866-78
The Journal of biological chemistry 2015 Dec 25;290(52):30866-78
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of MAPK8 over-expressed 293 cell line, cotransfected with MAPK8 Validated Chimera RNAi or non-transfected control. Blot probed with H00005599-M11. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of MAPK8 expression in transfected 293T cell line by MAPK8 monoclonal antibody (M11), clone 2F11. Lane 1: MAPK8 transfected lysatE (48.3 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: JNK1 Antibody (2F11) [H00005599-M11] - Detection limit for recombinant GST tagged MAPK8 is 1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of monoclonal antibody to MAPK8 on HeLa cell. Antibody concentration 10 ug/ml
