NBP2-67514

antibody from Novus Biologicals

Targeting: MAPK8 JNK, JNK1, PRKM8, SAPK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-67514

Provider

Novus Biologicals

Product name

Rabbit Monoclonal JNK1 Antibody

Antibody type

Monoclonal

Description

Protein A purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of Phospho-JNK1/2/3(T183+T183+T221) on different lysates using anti-Phospho-JNK1/2/3(T183+T183+T221) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 cell lysate, treated with Anisomycin Lane 2: NIH/3T3 cell lysate, untreated

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of paraffin-embedded human liver tissue using anti-Phospho-JNK1/2/3(T183+T183+T221) antibody. Counter stained with hematoxylin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of paraffin-embedded mouse heart tissue using anti-Phospho-JNK1/2/3(T183+T183+T221) antibody. Counter stained with hematoxylin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of paraffin-embedded human uterus tissue using anti-Phospho-JNK1/2/3(T183+T183+T221) antibody. Counter stained with hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of Hela cells with Phospho-JNK1/2/3(T183+T183+T221) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the second