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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- HPA019149 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA019149, RRID:AB_1852053
- Product name
- Anti-JUNB
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human JUNB, Gene description: jun B proto-oncogene, Validated applications: WB, IHC, ICC, ChIP, Uniprot ID: P17275, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Profound gene expression changes in the epithelial monolayer of active ulcerative colitis and Crohn’s disease
Xue X, Sæterstad S, Østvik A, Røyset E, Bakke I, Sandvik A, Granlund A
PLOS ONE 2022;17(3):e0265189
PLOS ONE 2022;17(3):e0265189
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human fallopian tube and skeletal muscle tissues using HPA019149 antibody. Corresponding JUNB RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- ChIP-Exo-Seq composite graph for Anti-JUNB (HPA019149, Lot C115965) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
