Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BS2646 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS2646, RRID:AB_1662615
- Product name
- Glucosidase IIÉÇ (K117) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 90-130 of Human Glucosidase IIÉÇ.
- Description
- Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of Glucosidases I and II in the endoplasmic reticulum (ER). Glucosidase II is an ER-localized enzyme that contains Ï« and ÉÇ subunits (Glucosidase IIÏ« and Glucosidase IIÉÇ). The Ï« and ÉÇ subunits form a defined heterodimeric complex with a molecular weight about 161 kDa. Glucosidase IIÏ« is the catalyitc core of the enzyme and can function independently of the ÉÇ subunit. The sequence of Glucosidase IIÉÇ encodes protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C terminus. The phosphorylated form of Glucosidase IIÉÇ is localized in the plasma membrane and is highly expressed in FGF stimulated fibroblasts and epidermal carcinoma cells. Glucosidase IIÉÇ was first purified from a human carcinoma cell line as a potential substrate for protein kinase C. Through the HDEL signal at the C-terminus, Glucosidase IIÉÇ retains the complete complex in the ER.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of Glucosidase II¦Â (K117) pAb at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:NIH-3T3 whole cell lysateLane3:PC12 whole cell lysate