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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX101856 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101856, RRID:AB_11172991
- Product name
- PRKCSH antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A549 B: HeLa 7.5% SDS PAGE GTX101856 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and PRKCSH knockout (KO) 293T cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRKCSH antibody (GTX101856) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PRKCSH antibody detects PRKCSH protein at endoplasmic reticulum by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PRKCSH protein stained by PRKCSH antibody (GTX101856) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PRKCSH antibody detects PRKCSH protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. PRKCSH antibody (GTX101856) dilution: 1:500.
