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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- PA5-21398 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRKCSH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2. Predicted reactivity: Mouse (86%), Rat (87%), Pig (90%), Bovine (88%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.94 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PRKCSH Polyclonal Antibody (Product # PA5-21398). Sample (30 µg of whole cell lysate). Lane A: A431 . 7.5% SDS PAGE. PRKCSH Polyclonal Antibody (Product # PA5-21398) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PRKCSH in paraformaldehyde-fixed A549 cells using a PRKCSH polyclonal antibody (Product # PA5-21398) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A549, using PRKCSH antibody (Product # PA5-21398) at 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRKCSH Polyclonal Antibody detects PRKCSH protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRKCSH protein stained by PRKCSH Polyclonal Antibody (Product # PA5-21398) diluted at 1:1,000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRKCSH Polyclonal Antibody detects PRKCSH protein at cytoplasm on human normal liver by immunohistochemical analysis. Sample: Paraffin-embedded normal liver. PRKCSH Polyclonal Antibody (Product # PA5-21398) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
