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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ8021 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PRKCSH Antibody / Glucosidase 2 subunit beta
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Glucosidase 2 subunit beta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human 293T, 3) human Daudi, 4) human HEL, 5) rat liver, 6) rat L6, 7) mouse liver and 8) mouse C2C12 cell lysate with Glucosidase 2 subunit beta antibody. Predicted molcular weight ~59 kDa but may be observed at higher molecular weights due to glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human A549 cells with Glucosidase 2 subunit beta antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human SiHa cells with Glucosidase 2 subunit beta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Glucosidase 2 subunit beta antibody.
