MA5-15873

antibody from Invitrogen Antibodies

Targeting: XRCC5 KARP-1, KU80, Ku86, KUB2

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15873

Provider

Invitrogen Antibodies

Product name

Ku80 Monoclonal Antibody (5C5)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15873 targets XRCC5 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human and mouse samples. The MA5-15873 immunogen is purified recombinant fragment of human XRCC5 expressed in E. Coli. . MA5-15873 detects XRCC5 which has a predicted molecular weight of approximately 86kDa.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

5C5

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

CAPRI enables comparison of evolutionarily conserved RNA interacting regions.

Panhale A, Richter FM, Ramírez F, Shvedunova M, Manke T, Mittler G, Akhtar A
Nature communications 2019 Jun 18;10(1):2682

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Lysine-specific demethylase 2A enhances binding of various nuclear factors to CpG-rich genomic DNAs by action of its CXXC-PHD domain.

Iuchi S, Paulo JA
Scientific reports 2019 Apr 2;9(1):5496

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Tyrosine kinase inhibitor-induced defects in DNA repair sensitize FLT3(ITD)-positive leukemia cells to PARP1 inhibitors.

Maifrede S, Nieborowska-Skorska M, Sullivan-Reed K, Dasgupta Y, Podszywalow-Bartnicka P, Le BV, Solecka M, Lian Z, Belyaeva EA, Nersesyan A, Machnicki MM, Toma M, Chatain N, Rydzanicz M, Zhao H, Jelinek J, Piwocka K, Sliwinski T, Stoklosa T, Ploski R, Fischer T, Sykes SM, Koschmieder S, Bullinger L, Valent P, Wasik MA, Huang J, Skorski T
Blood 2018 Jul 5;132(1):67-77

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Gene expression and mutation-guided synthetic lethality eradicates proliferating and quiescent leukemia cells.

Nieborowska-Skorska M, Sullivan K, Dasgupta Y, Podszywalow-Bartnicka P, Hoser G, Maifrede S, Martinez E, Di Marcantonio D, Bolton-Gillespie E, Cramer-Morales K, Lee J, Li M, Slupianek A, Gritsyuk D, Cerny-Reiterer S, Seferynska I, Stoklosa T, Bullinger L, Zhao H, Gorbunova V, Piwocka K, Valent P, Civin CI, Muschen M, Dick JE, Wang JC, Bhatia S, Bhatia R, Eppert K, Minden MD, Sykes SM, Skorski T
The Journal of clinical investigation 2017 Jun 1;127(6):2392-2406

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IGH/MYC Translocation Associates with BRCA2 Deficiency and Synthetic Lethality to PARP1 Inhibitors.

Maifrede S, Martin K, Podszywalow-Bartnicka P, Sullivan-Reed K, Langer SK, Nejati R, Dasgupta Y, Hulse M, Gritsyuk D, Nieborowska-Skorska M, Lupey-Green LN, Zhao H, Piwocka K, Wasik MA, Tempera I, Skorski T
Molecular cancer research : MCR 2017 Aug;15(8):967-972

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using XRCC5 monoclonal antibody (Product # MA5-15873) (Green). Red: actin filaments have been labeled with phalloidin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HeLa cells using XRCC5 monoclonal antibody (Product # MA5-15873) (green) and negative control (purple).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Interaction of N782 with the XRCC5/6 complex. * Indicates EGFP tag. ( a ) Left panel shows proteins immunoprecipitated with a rabbit anti-GFP antibody (black circle) and co-immunoprecipitated proteins (arrow). It contains both rabbit IgG heavy chain (50 kD wide band) and the light chain (25 kD wide band) derived from the anti-GFP antibody. Right panel shows Western blots of immunoprecipitated EGFP-KDM2A, EGFP-N782, and EGFP-KDM2A C-termini. The proteins were detected by a combination of mouse anti-GFP (primary) and goat anti-mouse IgG. ( b ) Keratinocyte genomic DNA fragments co-immunoprecipitated with EGFP-N782 and EGFP. A marker (250 or 500 ng) or sample (5 mul) was applied to each well, respectively. ( c ) Mass spectrometry of proteins included in the 70-90 kDa protein bands [proteins from top to bottom, (XRCC5 and XRCC6 in red) and (TMPO and LMNA in blue)]. ( d ) Western blotting analysis of XRCC5 (arrow) co-immunoprecipitated with N782 or the mutants (circle): Delta = deletion, C = CXXC, J = JmjC, and P = PHD. ( e ) Binding of GST-CN, GST-PN, and GST-NLSR to 3-length IGFBPL1 baits. Full-length blots are presented in Supplementary Fig. S1 . ( f ) Far Western blotting analysis of the interaction of KMD2A domains with XRCC5 and 6. Probe and protein dye are on the left; cross-reaction of antibody to an E. coli protein, > and