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Western blot
Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [2]
- Other assay [1]
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- Product number
- PA5-47149 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL13RA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinat human (rh) IL-13 R alpha 2, recombinant mouse IL-13 R alpha 1, rhIL-5 R alpha, rhIL-5 R beta, rhIL-4 R, and rhIL-9 R is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of recombinant Human IL-13 stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-13 Rα1 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47149). The ND50 is typically 10-30 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of IL13RA1 in Human whole blood granulocytes. Samples were incubated in IL13RA1 polyclonal antibody (Product # PA5-47149) or control antibody followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of IL13RA1 in TF‚1 human erythroleukemic cell line. Samples were incubated in IL13RA1 polyclonal antibody (Product # PA5-47149). Recombinant Human IL‚13 stimulates proliferation in the TF‚1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‚13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‚13 Rα1 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 10‚30 µg/mL.
