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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [3]
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- Product number
- PA5-28309 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL13RA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: U87-MG, U87-MG membrane extract, IL13RA1-transfected 293T. Predicted reactivity: Pig (80%), Bovine (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.51 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL13RA1/IL-12 Receptor alpha-1 using 30 µg of A) Jurkat (B) Raji and C) K562 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an IL13RA1/IL-12 Receptor alpha-1 polyclonal antibody (Product # PA5-28309) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IL13RA1 was performed by separating 30 µg of U87-MG whole cell and membrane extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a IL13RA1 Polyclonal Antibody (Product # PA5-28309) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IL13RA1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a IL13RA1 Polyclonal Antibody (Product # PA5-28309) at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IL13RA1 Polyclonal Antibody detects IL13 Receptor alpha 1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. IL13 Receptor alpha 1 stained by IL13RA1 Polyclonal Antibody (Product # PA5-28309) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IL13RA1 Polyclonal Antibody detects IL13 Receptor alpha 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. IL13 Receptor alpha 1 stained by IL13RA1 Polyclonal Antibody (Product # PA5-28309) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using IL13 Receptor alpha 1 (Product # PA5-28309) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
