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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
- Functional assay [1]
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- Product number
- DM1227 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#DM1227, RRID:AB_10655127
- Product name
- anti CD213a1 / IL13RA1 (Extracell. Dom.)
- Antibody type
- Monoclonal
- Antigen
- Genetic immunization with cDNA encoding Human IL13-R alpha 1 (extracellular domain).Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GM1E7
- Vial size
- 0.1 mg
- Concentration
- 1.5 mg/ml
Submitted references Blockade of interleukin-13-mediated cell activation by a novel inhibitory antibody to human IL-13 receptor alpha1.
Krause S, Behrends J, Borowski A, Lohrmann J, Lang S, Myrtek D, Lorenzen T, Virchow JC, Luttmann W, Friedrich K
Molecular immunology 2006 Apr;43(11):1799-807
Molecular immunology 2006 Apr;43(11):1799-807
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 2: Spectral Confocal Microscopy of CHO cells using GM-1E7 antibody (Cat.#DM1227). CHO cells were transiently transfected with an expression vector encoding IL13-R. Binding of GM-1E7 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (Red). Cell nuclei are stained with DAPI (Blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1: FACS analysis of BOSC23 cells using GM-1E7 antibody (Cat.#DM1227). BOSC23 cells were transiently transfected with an expression vector encodingeither IL13-R (Red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-1E7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with IL13-R transfected cells.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 3: Antagonistic Properties of GM-1E7 Dose-dependent inhibition of cellular proliferation. Samples of TF-1 cells were incubated for 72 h with 10 nM human IL-13 (closed circles) or 10 nM Human IL-4 (open circles) plus the indicated concentrations of purified GM-1E7. Antibody GM1E7 blocks IL-13-dependent cell proliferation completely, but only marginally influences IL-4-activity on TF-1 cells.
