Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00005048-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005048-M03, RRID:AB_2236701
- Product name
- Mouse Monoclonal LIS1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PAFAH1B1 - platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa (5A5)
- Reactivity
- Human, Mouse, Bovine, Porcine, Sheep
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LIS1 Antibody (5A5) [H00005048-M03] - Analysis of PAFAH1B1 expression in transfected 293T cell line by PAFAH1B1 monoclonal antibody (M03), clone 5A5. Lane 1: PAFAH1B1 transfected lysatE (46.6 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: LIS1 Antibody (5A5) [H00005048-M03] - Detection limit for recombinant GST tagged PAFAH1B1 is 0.03 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: LIS1 Antibody (5A5) [H00005048-M03] - Analysis of monoclonal antibody to PAFAH1B1 on HeLa cell. Antibody concentration 15 ug/ml